Do you need to have at least 1 Insight to level up?

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I know that when you receive your first Insight you are given access to the level up NPC in the Hunter's Dream. If you ever go back down to 0 Insight do you lose the ability to level up again?
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Is Insight important in Bloodborne?
Insight is a stat in Bloodborne. It governs the amount of inhuman knowledge you have acquired by exploring the game and defeating enemies. It is required to use the Beckoning Bell to summon other players into your game world.Does Insight affect difficulty?
Higher Insight levels will increase the difficulty of the game because enemies will gain new and more powerful attacks. The player's vulnerability to Frenzy will also increase drastically.How much Insight do you need for a Doll?
One Insight is required to initially interact with the Doll. If the player's Insight drops back down to zero, she will become inanimate again upon appearing in the Hunter's Dream, although it will still be possible to interact with her in order to level up.Bloodborne - How To Level Up/Fastest Insight
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