How to use the Tiny Music Box?

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I have a Tiny Music Box, which I have been trying out with a few different enemies. I noticed it doesn't seem to affect normal enemies, nor the first boss, Cleric Beast.
It looks like it affected the 2nd boss, Father Gascoigne, though. He covered his ears for a brief second, but I wasn't close enough to attack him afterwards.
I wonder if someone can shed some light on how to effectively use this item?
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Does the tiny music box only work once?
You'll only be able to get around three uses of the music box in before Father Gascoigne enrages and becomes a beast. It happens when he's at around 50 per cent health, so time your music box uses carefully to get the most benefit before this happens. After he's transformed, you're allowed to use it just once more.What happens if I summon Father Gascoigne?
If you summon Father Gascoigne for the Cleric Beast boss fight and you happen to have the Tiny Music Box, using it will make Father Gascoigne give you an unimpressed grunt or a small chuckle. Gascoigne's surname is based off Irish origin.Does the music box work in Father Gascoigne?
Equip this music box in your item slots and head for Father Gascoigne. Once you're in the fight, the music box can be used three times to stagger him. Keep in mind that it makes him turn into a werewolf sooner, so is worth saving until his second stage at the earliest when he extends his axe.Does the music box work on Vicar Amelia?
Defeating Vicar Amelia renders this item unobtainable.Bloodborne - Father Gascoigne Boss Fight + Using Tiny Music Box Makes For A Fast Fight
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Images: Pixabay, Andrea Piacquadio, Andrea Piacquadio, Mike B